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Canine distemper virus symptom
Description
Canine distemper is a highly contagious and fatal infectious disease caused by the canine distemper virus. Clinical features were biphasic heat type. The digestive tract, respiratory tract mucosa with acute catarrhal inflammation, late non suppurative encephalitis. Occurs in the 3-6 month old puppies and young dogs are also infected, the incubation period is 3-6 days. At the beginning of the disease manifested as temperature increased 39.5-41.0, loss of appetite, mild mental depression, eyes, nose outflow pus like discharge, sneezing, temporarily diarrhea. After 2-3 days in the asymptomatic stage. After 2-14 days again increased body temperature and clinical symptoms, fever continued for several weeks, a fever type. Bilateral conjunctivitis or keratitis, mucus or purulent mucus flow. In some cases of severe disease in dogs may corneal ulcer and perforation can lead to blindness. Virus alone infection symptom is slight, if the symptom of secondary bacteria infection is aggravating, the respiratory system symptom is more serious in winter.
The detection principle of
Test paper for the immune chromatography (double anti sandwich method) qualitative detection of canine eye secretions (or conjunctival), nose, saliva, genital and anal (feces) and serum CDV antigen. In the window of the detection card judgment judgment line "T line" detection and control line "C line". Before adding the sample, the "T line" and "C line" do not show. "C line" is used to confirm whether the sample is normal in the kit. "T line" is used to confirm whether the sample contains CDV antigen.
The composition of the product
Card (with disposable pipette and desiccant) 10. With sample dilution tube pipe 10 3. Odd sterilization packaging samples instructions collected swab 10. A 1. CDV Ag strip
The preservation and duration of
The reagent box is kept in a cool and dry place at 2-30 degrees C, and can not be frozen. Avoid direct sunlight, the production date is valid for 18 months. Open the test card packaging, use within 1 hours. Sample requirements
1 the test paper samples for plasma / serum, eye and conjunctival secretions, nasal fluid, saliva, urine.
2. Samples pay attention to multiple sites also collected fresh and valid samples, fully in a test tube mixing dilution, to obtain supernatant. 3 samples must be immediately detected, otherwise it should be kept refrigerated for more than 24 hours, should be kept refrigerated.
The detection step
1 with saline wet swabs collected from dogs eye and conjunctival secretions, nasal fluid, saliva, urine. If serum / plasma directly using the dropper (add 1 drop of blood serum, add 3 drops of diluent). 2 will invade with cotton swab sample dilution tube, fully mixing, the supernatant with a disposable burette. 3 out strip, Kaifeng flat on the desktop, from the dropper slowly and accurately by adding 2-3 drops of liquid drops. 4 5 minutes and 10 minutes after the judgment result, the result is invalid.
2016.2.1
